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. 2017 Sep 15;8(9):228. doi: 10.3390/genes8090228

Figure 2.

Figure 2

Figure 2

Performance assessment of P. ginseng Iso-Seq. (A) CEGMA evaluation. The completeness (%) of de novo assemblies from Iso-Seq and RNA-Seq data were assessed by CEGMA. In the result, RNA-Seq dataset 1 and dataset 2 unigenes were generated from data by Wang et al. (2016) [13] and from our unpublished leaf RNA-Seq data, respectively. (B) Validation of an unigene by Sanger sequencing. Sequence identity between unigene KG_ISO_000349 and the corresponding Sanger sequence was examined using zPicture. (C) Performance assessment of P. ginseng Iso-Seq. Mapping of RNA-seq dataset 1, derived from 16 tissues, to the two unigene sets assembled from Iso-Seq and RNA-Seq dataset 1 of P. ginseng using BWA.