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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: Nano Lett. 2017 Jun 26;17(7):4427–4435. doi: 10.1021/acs.nanolett.7b01571

Figure 4.

Figure 4

(A, B) Western blot analysis of BRD4 expression in LNCaP cells treated with siBRD4-loaded TCPA2-NPs at pH 6.8 (A) and 7.4 (B). (C-E) Immunofluorescence analysis of BRD4 expression (red fluorescence) in LNCaP cells treated with siBRD4-loaded TCPA2-NPs (20 nM siRNA dose) at pH 6.8 vs. 7.4. Blank: cells incubated in free culture medium. (F) Flow cytometry analysis and (G) quantification of apoptosis of LNCaP cells treated with siBRD4-loaded TCPA2-NPs at a 20 nM siRNA dose. (H) Proliferation profile of LNCaP cells treated with siBRD4-loaded TCPA2-NPs at a 20 nM siRNA dose. The siLuc-loaded TCPA2-NPs were used as the control in these experiments.