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(A, B) Western blot and (C, D) immunohistochemistry analysis of BRD4 expression in the LNCaP tumor tissue after systemic treatment by control NPs and siBRD4-loaded TCPA2-NPs. *** P < 0.001 (E, F) TUNEL staining of the LNCaP tumor tissue after systemic treatment by control NPs and siBRD4 TCPA2-NPs. TUNEL-positive apoptotic cells were stained with red fluorescence. The siLuc-loaded TCPA2-NPs were used as control NPs.
