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. 2017 Sep 22;8:116. doi: 10.3389/fgene.2017.00116

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Copyright © 2017 Kandhro, Shoombuatong, Nantasenamat, Prachayasittikul and Nuchnoi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Defined number of predicted and validated target genes from Top 20 significant miRNAs. Among the 20 significant miRNAs, only 18 showed a defined number of targets at threshold 3 functionality modes on CyTargetLinker and Cytoscape. The miRNAs and their accession numbers are in red circles; pink hexagonal shapes indicate their regulatory interaction (targeted mRNAs/genes). The different colors of arrows show regulatory interactions for the identification based on miRNA validated and predicted databases (miRTarBase, MicroCosm, and TargetScan). Each miRNA targets mRNA/genes and the high number of such targets is shown in (C) where miRNA-155 has 31 targets and miRNA-200c has 11. In (D) miRNA-103 has 11 targets. Single targets also were found as shown in (A) for miRNA-144 and (B) for miRNA-33b and miRNA-185.