Fig. 3.

DIO prevents ADR-triggered DNA damage in RPE cells.

(A) Immunofluorescent micrographs of control and drug-treated (24 h) cells stained for γ-H2AX foci (blue—nucleus; green—γ-H2AX foci). (B) Quantitation of the γ-H2AX foci in the micrographs from A. The data represent the mean ± SD (n = 4), ^***P < 0.001 (ADR vs. control), ^††P < 0.01 (DIO + ADR vs. ADR). (C) In vitro western blot analysis of p53 and γ-H2AX expression in control and drug-treated (24 h) lysates. In vivo western blot analysis of γ-H2AX expression in control and drug-treated lysates.