Fig. 5.

DIO Inhibits ADR-induced mitochondria dysfunction in RPE cells.

(A) The effect of DIO on loss of the mitochondrial membrane potential in RPE cells. After treatment with DIO and ADR for 24 h at the indicated concentrations, a loss in the mitochondrial membrane potential was observed by microscopy after JC-1 staining. The green fluorescence intensity indicated the cells with low mitochondrial membrane potential, while the red fluorescence intensity indicated the cells with stable mitochondrial membrane potential (n = 4). (B) Western blot analysis and corresponding densitometric measurements of the mitochondrial marker, Bcl-2. Effect of DIO on Bcl-2 protein expression induced by ADR in cultured RPE cells. Cells were incubated with 1.5 μM ADR and 6 μM DIO for 24 h and lysed; Bcl-2 was analyzed by western blot. The data represent the mean ± SD (n = 4), ^*P < 0.05 (ADR vs. control), ^†P < 0.05 (DIO + ADR vs. ADR).