Fig. 4.

Effects of pH and temperature on the enzyme activity of purified rPcAxe with 4-nitrophenyl-α-l-arabinofuranoside. a Effect of temperature on enzyme activity. The optimal temperature was determined in the range of 20–60 °C. b Effect of pH on enzyme activity. Enzyme activity was measured between pH 5 and 11 using mixtures of 0.1 M citric acid and 0.2 M sodium hydrogen phosphate. The maximum activity was defined as 100%, and the relative activity was determined. Values are the means and standard deviations of triplicate experiments