Fig. 5.
Nocodazole induces expression of TvCMD1 in a time-dependent manner. a Western blotting revealed the expression of TvCMD1 after nocodazole (10 μM) treatment for 0, 3 and 6 h. The iron-rich cultured cells were synchronized by cold incubation for 3 h prior to nocodazole addition (cold). GAPDH was used as the loading control. b The quantified result of nocodazole-treated groups based on penal (a). Asterisks represent the significance of 6 h compared to 0 h as determined through P-value (**P < 0.01). The expression of TvCMD1 could be induced by the treatment of nocodazole, the G2/M inhibitor. Abbreviation: IR, iron-rich (negative control)