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. 2017 Sep 26;10:443. doi: 10.1186/s13071-017-2381-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Nocodazole induces expression of TvCMD1 in a time-dependent manner. a Western blotting revealed the expression of TvCMD1 after nocodazole (10 μM) treatment for 0, 3 and 6 h. The iron-rich cultured cells were synchronized by cold incubation for 3 h prior to nocodazole addition (cold). GAPDH was used as the loading control. b The quantified result of nocodazole-treated groups based on penal (a). Asterisks represent the significance of 6 h compared to 0 h as determined through P-value (**P < 0.01). The expression of TvCMD1 could be induced by the treatment of nocodazole, the G2/M inhibitor. Abbreviation: IR, iron-rich (negative control)