Table 1.

Oligonucleotides used

Name	Sequence 5′–3′	Use
LS-005	TTTTTTCATATGTCCATCACCGCCAGCGAAG	Forward (Fd) for yefMsl amplification. NdeI sequence is underlined
LS-007	TTTTTTAAGCTTCACGCCCGCTCCGCGTCCG	Reverse (Rev) for yefMsl amplification. HindIII sequence is underlined
LS-008	TTTTTTCATATGAGGATCACTTTCACGTCCCAC	Fd for yoeBsl amplification. NdeI sequence is underlined
LS-009	TTTTTTCTCGAGTCAGTAGTGGTAGCGCGCCTGG	Rev for yoeBsl amplification. XhoI sequence is underlined
LS-113	CGACTGATGTCATCAGCGGTGG	Fd for aac(3′)IV (apramycin resistance gene) amplification
LS-114	CCAACGTCATCTCGTTCTCCGC	Rev for aac(3′)IV (apramycin resistance gene) amplification
LS-124	ATGATTGAACAAGATGGATTGCACG	Fd for aph(3′) (neomycin resistance gene) amplification
LS-125	TCAGAAGAACTCGTCAAGAAGGCG	Rev for aph(3′) (neomycin resistance gene) amplification
MRG-11	TTTTTTCATATGGCCCGCAGACTCCGCACC	Fd for amy amplification. NdeI sequence is underlined
MRG-12	TTTTTTCTCGAGGCCGCGCCAGGTGTCGTTGAG	Rev for amy amplification. XhoI sequence is underlined
LS-116	CATATGGCTCAGAATCCCCCGG	Fd for xysA amplification
LS-117	CTCGAGCGCGGCGAGCACCG	Rev for xysA amplification