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. 2017 Sep 26;36:133. doi: 10.1186/s13046-017-0601-6

Fig. 6.

Fig. 6

RSV induces EBV into lytic phase in NKTCL cells and has no effect on the expression of LMP1 in mRNA and protein levels. a The mRNA level of EBV lytic gene Zta in cells after treated with RSV for different hours was measured using Real-time PCR assay (n = 3).*p < 0.05, # p < 0.01 vs control. b Protein level of Zta in cells was determined by western blot analysis. Cells were treated with 25 μM RSV for different hours (n = 3). c Protein level of Zta was detected by immunofluorescence in cells incubated with RSV for 6 h. Cell nuclei were stained by DAPI. Images were acquired using confocal laser-scanning microscope (Scale bar, 20 μm). d The mRNA level of LMP1 in cells after treated with RSV for different hours (n = 3).*p < 0.05, # p < 0.01 vs control. e Protein level of LMP1 in cells after treated with RSV in different concentration at 48 h