Table 1.
Primers and amplicons for detecting ETFDH gene mutation
| Name | Sequence of primers | Amplicon(bp) |
|---|---|---|
| ETFDH-Exon1-F | CAATCGATCTCGAAGGGCACT | 659 bp |
| ETFDH--Exon1-R | TTAATTCTACCAACTGGGGCAAC | |
| ETFDH--Exon2-F | GTTAAGCCTGACATGAGCTAAATTG | 537 bp |
| ETFDH--Exon2-R | TAATTGTCGTGTTGTTGACTGAAGG | |
| ETFDH--Exon3-F | TGTGCAAAACACAGGGAGAATTTC | 512 bp |
| ETFDH--Exon3-R | AGCCTGGGCAACAAGAGTGAAA | |
| ETFDH--Exon4-F | AGGAGAAACACTTGAACCCAGGA | 502 bp |
| ETFDH--Exon4-R | AGTAAGTCCTTCAAATATCTGGGTCTC | |
| ETFDH--Exon5-F | TGAAAGTGTGACCATCAATGTAGCA | 437 bp |
| ETFDH--Exon5-R | TTGATGGGGGTACAACATGAGGA | |
| ETFDH--Exon6-F | CTTTCTCATCAAGGTTGTGGCAT | 405 bp |
| ETFDH--Exon6-R | TTGGAGAGATGGGGTTTCACTTT | |
| ETFDH--Exon7-F | CCATTGGCAGAGGAGCTGGAT | 683 bp |
| ETFDH--Exon7-R | CAACACTTGATACGACAATTCCAAC | |
| ETFDH--Exon8-F | CACCGTGCCCAGCCTTCTT | 477 bp |
| ETFDH--Exon8-R | AATGACACAGCAGCCATAAGCACT | |
| ETFDH--Exon9-F | CCAGAGCACAAGGATTTCTTAATATG | 693 bp |
| ETFDH--Exon9-R | ATTTGTTGTAGAGATGGGGTTTCG | |
| ETFDH—Exon10-F | ATTTTTCAGCCTTTCCCTACAGC | 551 bp |
| ETFDH—Exon10-R | GCCTACCAAAGTGCTGGGATTAC | |
| ETFDH—Exon11-F | CCAACCTGGGTGACAGAGCAA | 580 bp |
| ETFDH—Exon11-R | CTTCAACAATTTTGAGGGAAATGCT | |
| ETFDH—Exon12-F | TGAGGGCTAGTCATATTTCTTTGGT | 444 bp |
| ETFDH—Exon12-R | TTTCTAAGGAATGGAAGGAGATACAG | |
| ETFDH—Exon13-F | TGAGAGGATGACTGTGAATAAGGGA | 689 bp |
| ETFDH—Exon13-R | GAACTGAAGAGGTAGGAAGATGCTG |