Table 2. Uba1 Adenylation Activity Michaelis-Menten Values.

	Vmax (Ub)	95% CI	Km (Ub)	95% CI
WT	0.04063	0.03337 to 0.04789	19.89	9.507 to 30.27
p.M539I	0.03382	0.03117 to 0.03647	9.405	6.449 to 12.36
p.S547G	0.03763	0.02887 to 0.04639	28.72	12.12 to 45.32
p.E557V	0.0285	0.02362 to 0.03338	16.72	7.894 to 25.55
	Vmax (ATP)	95% CI	Km (ATP)	95% CI
WT	0.04346	0.04069 to 0.04623	7.491	4.670 to 10.31
p.M539I	0.0384	0.03619 to 0.04062	4.324	2.754 to 5.894
p.S547G	0.03461	0.03129 to 0.03793	8.547	3.724 to 13.37
p.E557V	0.03372	0.02964 to 0.03780	29.07	13.63 to 44.50