Skip to main content
. 2016 Jan 13;3:225–229. doi: 10.1016/j.toxrep.2016.01.002

Fig. 1.

Fig. 1

Nested polymerase chain reaction (n-PCR) detection of t(14:18) IGH:BCL2 in DNA from farmers and controls. (A) Representative electrophoretic separation of PCR products from the second round of n-PCR using primers specific to the major break point (MBR) region. The primers detect products ranging from 80 to 300 base pairs (bp) in the MBR region. Products are seen in 4 out of 5 samples from farmers and the positive control, but not in the negative control or control samples. (B) Representative electrophoretic separation of PCR products from participant samples and water (negative) control for internal control β-Globin. A band measuring 268 bp is seen for the participant samples but not the negative control. (C) Quantification of the percentage of t(14:18) IGH:BCL2 positivity showing a significant increase in the percentage of t(14:18) IGH:BCL2 positivity in farmers (63.5%) compared to controls (11.5%), with an odds ratio (OR) = 13.5; 95%, and a confidence interval (CI) = 6.3–28.6. ****p < 0.0001 calculated using Pearson Chi-Square test.