Table 1.
List of primers and PCR amplification conditions used for VSX1 gene amplification
| Exon | Sequence of primers (5′-3′) | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|
| 1 | CAGCTGATTGGAGCCCTTC(sense) | 62 | 599 |
| CTCAGAGCCTAGGGGACAGG(antisense) | |||
| 2 | CTTAAGTACCCAAGAGGTTCATAACT(sense) | 62 | 269 |
| GAAACCACTGGGCCTGCTATCAT(antisense) | |||
| 3 | AAGCAGGCACGGTGGTCCTTA(sense) | 63 | 293 |
| AAGGGACTGCTGATTGGCTCACT(antisense) | |||
| 4 | GCTCGGGAGAGAAGATCC(sense) | 62 | 399 |
| TCAGTAAACTGACGTTGCTTG(antisense) | |||
| 5 | GGAAATTTACTTCATTGCTGAATTT(sense) | 60 | 495 |
| TGGCATTGCATTTTATCTTGACA(antisense) |