Table 1.
Plasmids and strains used in this study
| Plasmid or strain | Genotype | Source or reference |
|---|---|---|
| Plasmids | ||
| YCplac22 | Amp Trp1 | Gietz [50] |
| YCplac33 | AmpURA3 | Gietz [50] |
| Y22-T4 | TADH1/TTDH3 | This work |
| Y22-AtF3H | PPGK1-AtF3H-TADH1 | This work |
| Y22-T4-AtF3H-AtFLS | PPGK1-AtF3H-TADH1/PTDH3-AtFLS-TTDH1 | This work |
| Y22-T4-AtF3H-CitFLS | PPGK1-AtF3H-TADH1/PTDH3-CitFLS-TTDH1 | This work |
| Y22-T4-AtF3H-MdFLS | PPGK1-AtF3H-TADH1/PTDH3-MdFLS-TTDH1 | This work |
| Y22-T4-AtF3H-PdFLS | PPGK1-AtF3H-TADH1/PTDH3-PdFLS-TTDH1 | This work |
| Y22-T4-AtF3H-ZmFLS | PPGK1-AtF3H-TADH1/PTDH3-ZmFLS-TTDH1 | This work |
| Y33-ALAC-ADH2 | PTEF2-ACS SE-TRPS2/PPGK1-ALD6-TTDH1/PHXT7-ADH2-TRPL9A | This work |
| Y33-ALAC-ACAD | PTEF2-ACS SE-TRPS2/PPGK1-ALD6-TTDH1/PHXT7-ADH2-TRPL9A/PPGK1-ACC1-TCCW12 | This work |
| Strains | ||
| W303-1A | MATa leu2-3112 ura3-1 trp1-92 his3-11,15 ade2-1 can1-100 | Thomas [51] |
| W3-AtF3H | W303-1A carrying Y22-AtF3H | This work |
| W3-AtFLS | W303-1A carrying Y22-T4-AtF3H-AtFLS | This work |
| W3-CitFLS | W303-1A carrying Y22-T4-AtF3H-CitFLS | This work |
| W3-MdFLS | W303-1A carrying Y22-T4-AtF3H-MdFLS | This work |
| W3-PdFLS | W303-1A carrying Y22-T4-AtF3H-PdFLS | This work |
| W3-ZmFLS | W303-1A carrying Y22-T4-AtF3H-ZmFLS | This work |
| W3NP | YORWdelta17::His3/PADH1-4CL-TTPI1/PHXT7-CHS-TTPG1/PPGI1-CHI-TADH1/PADH1-PAL-TFBA1/PTDH3-C4H-TPDC1 | This work |
| W3NP-FF | W3NP carrying Y22-T4-AtF3H-PdFLS and YCplac33 | This work |
| W3NP-FF-A3 | W3NP carrying Y22-T4-AtF3H-PdFLS and Y33-ALAC-ADH2 | This work |
| W3NP-FF-A4 | W3NP carrying Y22-T4-AtF3H-PdFLS and Y33-ALAC-ACAD | This work |