Fig. 10.

TEER and cell viability of Calu-3 cell monolayers. Calu-3 cells were seeded on permeable filters as described in Section 2.12. After a tight monolayer had formed, MWCNT (80 μg/cm²) were added to the apical compartment, and exposure prolonged for up to 12 days. At the indicated times, TEER was determined, as described under Methods. (A) TEER (% of control) recorded every 3d up to 12d. (B) TEER (% of control) at day 12. (C) Cell viability was assessed in the same wells used for TEER determinations with the resazurin assay. For all panels, data are means ± S.D of 4 independent determinations. *p < 0.05 vs. control, untreated cultures. The experiment was performed twice with comparable results.