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. Author manuscript; available in PMC: 2018 Sep 13.
Published in final edited form as: Cell Host Microbe. 2017 Sep 13;22(3):377–386.e5. doi: 10.1016/j.chom.2017.08.004

Figure 4. Identification of m6A sites on IAV-PR8 minus strand vRNA.

Figure 4

PAR-CLIP and PA-m6A-seq were performed on RNA from 293T cell lines 24 hpi with IAV-PR8 at MOI of 10. (A) Concatenated map of the IAV-PR8 genome used for read alignment. (B) Complete genomic coverage tracks are shown, similar to Fig. 2. The PA-m6A-seq lane has a Y axis of 0–2000 reads, and all others are depicted with Y axes of 0–300 reads. (C) An expanded view of the HA segment of IAV-PR8, with 9 prominent m6A sites numbered. PA-m6A-seq has a Y axis of 0–1000 reads, and all others are depicted with Y axes of 0–150 reads. Green/brown bars indicate the level of A>G conversion at specific A residues.