Figure EV3. Translational activity in the fat body under reduced IIS is reduced in a dfoxo‐dependent manner.

• A–D
  De novo protein synthesis as measured by incorporation of ³⁵S into proteins from ex vivo (A) fat body, (B) head, (C) thorax and (D) gut tissue. Equal levels of protein were loaded per lane. Representative gel exposures show fat body ³⁵S incorporation from w ^Dah , dfoxo ^Δ94, InsP3‐Gal4/UAS‐rpr and InsP3‐Gal4/UAS‐rpr, dfoxo ^Δ94 flies. Quantification of gel exposures normalized to total protein and corresponding two‐way ANOVA, as well as Bonferroni corrected post hoc tests are shown alongside each gel exposure. Error bars show SEM, n = 11 for w ^Dah and InsP3‐Gal4/UAS‐rpr fat body samples, for all other genotypes and tissues n = 5, *P < 0.05, ***P < 0.001.