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AQP4 knockdown diminished the protective effect of miR-145 in astrocytes. (a, b) Western blotting identification of AQP4 siRNA efficiency; β-actin was used as the internal control. (c) ELISA of LDH levels in astrocyte culture supernatant. (d, e) Flow cytometry assay of astrocyte apoptosis. Cells in the B2 and B4 quadrants are apoptotic cells.
