. 2016 Aug 10;3:696–700. doi: 10.1016/j.toxrep.2016.08.002

Table 2.

Spermatozoa of D. rerio exposed to Beltsville Thawing Solution (BTS) alone (control) or 0.5, 5, or 10 μg/L cadmium at 20 °C for 10 min were evaluated for high fluidity (FLU) and integrity of the plasma membrane, high functionality mitochondrial (FMI), the sperm chromatin structure assay (SCSA) and the production of reactive oxygen species (ROS) (n = 10). * ROS expression is based on the median fluorescence intensity.

[Cd ⁺²]	SCSA	Membrane Integrity (%)	FMI (%)	FLU (%)	ROS
0 (BTS)	7.27 ± 1.0^A	83.40 ± 8.5^A	74.87 ± 8.3^A	81.60 ± 3.1^A	3428.8 ± 886.2^A
0.5	9.53 ± 1.2^A	73.93 ± 8.7^AB	69.04 ± 6.1^A	78.54 ± 3.7^A	2584.3 ± 654.5^A
5	13.72 ± 3.0^B	47.85 ± 9.0^B	65.82 ± 5.2^A	77.39 ± 4.3^A	2145.0 ± 356.9^A
10	14.29 ±3.3^B	41.52 ± 10.1^B	64.47 ± 5.8^A	77.11 ± 3.3^A	2279.5 ± 428.2^A

Data are expressed as the mean and standard error of the mean. Different letters in the same column indicate significant differences based on the nonparametric Kruskal-Wallis test with a 0.05 significance threshold.