Table 3.

Spleen and thymus B and T cell lymphocytes in adult female B6C3F1 mice following oropharyngeal aspiration exposure to CBN 3 geogenic dust each week for 28 days.

	Spleen
Geogenic dust (mg/kg)	CD4+ (cells × 107)	CD8+ (cells × 107)	CD4+/CD8+ (cells × 106)	CD4-/CD8- (cells × 108)	B220 (cells × 107)
0	4.09 ± 0.715	2.02 ± 0.422	1.36 ± 0.412	1.11 ± 0.213	5.78 ± 1.27
0.01	3.93 ± 0.440	1.93 ± 0.287	1.25 ± 0.301	1.08 ± 0.192	6.11 ± 1.78
0.1	3.70 ± 0.899	1.81 ± 0.358	1.24 ± 0.312	1.00 ± 0.181	5.35 ± 0.515
1	3.44 ± 1.22	1.76 ± 0.574	1.24 ± 0.474	1.05 ± 0.347	5.70 ± 2.91
10	2.91 ± 0.591*	1.43 ± 0.300*	0.850 ± 0.216*	0.869 ± 0.196	4.83 ± 1.36
100	2.90 ± 0.633*	1.50 ± 0.326*	0.797 ± 0.238*	0.888 ± 0.181	5.71 ± 0.877

	Thymus
Geogenic dust (mg/kg)	CD4+ (cells × 107)	CD8+ (cells × 106)	CD4+/CD8+ (cells × 107)	CD4-/CD8- (cells × 106)
0	1.60 ± 0.274	8.63 ± 1.44	11.54 ± 1.77	8.53 ± 1.64
0.01	1.19 ± 0.349	6.29 ± 1.65*	8.58 ± 1.85*	6.00 ± 1.41*
0.1	1.29 ± 0.166	6.42 ± 0.827	9.05 ± 0.738*	6.20 ± 1.29
1	1.50 ± 0.416	6.60 ± 1.96	9.71 ± 2.04	6.60 ± 1.32
10	1.20 ± 0.135	5.84 ± 0.971*	8.17 ± 1.08*	6.39 ± 1.25
100	1.08 ± 0.389*	4.89 ± 1.62*	6.96 ± 0.800*	5.59 ± 1.57*

	Spleen
Geogenic dust (mg/kg)	4+/25+/fp3+ (cells × 107)	4+/25-fp3+ (cells × 107)	4+/25+/fp3- (cells × 105)	4+/IL17A+ (cells × 106)	4-/IL17A+ (cells × 106)
0	1.74 ± 0.438	1.48 ± 0.505	6.38 ± 2.32	0.755 ± 0.274	0.960 ± 0.482
0.01	1.85 ± 0.364	1.72 ± 0.347	6.60 ± 2.42	1.31 ± 0.213	2.02 ± 0.458
0.1	1.69 ± 0.278	1.78 ± 0.426	5.96 ± 1.31	1.10 ± 0.172	1.23 ± 0.269
1	1.68 ± 0.484	1.73 ± 0.602	6.45 ± 3.12	0.755 ± 0.620	1.42 ± 0.418
10	1.59 ± 0.384	1.60 ± 0.390	6.37 ± 1.66	0.836 ± 0.219	1.34 ± 0.431
100	1.44 ± 0.297	1.28 ± 0.306	6.65 ± 2.04	0.718 ± 0.181	1.56 ± 0.144

Data are presented as mean cell number ± standard deviation. Sample size for each group was 5–6 animals and are representative of three trial days.

^*indicates aresponse statistically different from the 0 mg/kg group (p < 0.05) as determined from log transformed data.