Table 1.
List of 24 metabolites revealing with significant changes between the different treatment groups of HaCaT WT cells (p-values result from MANOVA; metabolite concentrations in nmol/g pellet weight ± SD). Abbreviations used: BAs = biogenic amines (alpha-AAA = a-aminoadipic acid); ACs = acylcarnitines (Cx:y = acylcarnitines, Cx:y-OH = hydroxyacylcarnitines, Cx:y-DC = dicarboxyacylcarnitines); AAs = amino acids (Gln = glutamine); PCs = phosphatidylcholines (PCaa_Cx:y = diacyl, PCae_Cx:y = acyl-alkyl); SMs = sphingomyelines (SM_Cx:y); x = number of carbon atoms in the lipid chain, y = number of double bonds.
| Metabolite | Category | Control | BAP | CRY | DALP | P value |
|---|---|---|---|---|---|---|
| alpha-AAA | BAs | 11.2 ± 0.9 | 5.7 ± 1.8 | 14.2 ± 3.8 | 2.0 ± 1.1 | 0.000 |
| C10:2 | ACs | 0.7 ± 0.1 | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.1 ± 0.1 | 0.001 |
| C12:1 | 0.9 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.2 | 1.4 ± 0.1 | 0.000 | |
| C12-DC | 2.9 ± 0.3 | 3.8 ± 0.2 | 3.3 ± 0.6 | 4.2 ± 0.2 | 0.001 | |
| C18:1-OH | 0.7 ± 0.1 | 0.9 ± 0.1 | 0.8 ± 0.1 | 1.0 ± 0.0 | 0.001 | |
| C6 (C4:1-DC) | 1.7 ± 0.3 | 2.3 ± 0.2 | 1.9 ± 0.3 | 2.5 ± 0.2 | 0.004 | |
| C18:2 | 0.7 ± 0.1 | 1.0 ± 0.1 | 0.8 ± 0.1 | 1.1 ± 0.1 | 0.000 | |
| C4 | 2.0 ± 0.3 | 2.5 ± 0.3 | 2.3 ± 0.5 | 2.9 ± 0.3 | 0.029 | |
| C4:1 | 0.8 ± 0.1 | 1.1 ± 0.1 | 0.9 ± 0.2 | 1.3 ± 0.1 | 0.001 | |
| Gln | AAs | 149.4 ± 12.4 | 77.1 ± 18.2 | 114.3 ± 12.8 | 65.7 ± 23.9 | 0.000 |
| PCaa_C32:2 | PCs (aa) | 109.8 ± 12.4 | 103.6 ± 11.5 | 127.8 ± 17.3 | 81.9 ± 9.5 | 0.003 |
| PCaa_C36:2 | 1644.0 ± 221.7 | 2039.2 ± 198.8 | 2097.1 ± 262.1 | 1417.7 ± 173.7 | 0.002 | |
| PCae_C32:1 | PCs (ae) | 196.3 ± 29.9 | 198.8 ± 17.1 | 231.2 ± 36.0 | 164.7 ± 17.4 | 0.029 |
| PCae_C34:2 | 224.4 ± 36.1 | 238.2 ± 23.8 | 275.4 ± 48.2 | 170.7 ± 25.4 | 0.009 | |
| PCae_C34:3 | 24.4 ± 3.0 | 26.8 ± 3.1 | 29.7 ± 5.0 | 20.8 ± 3.3 | 0.031 | |
| PCae_C36:2 | 309.1 ± 43.7 | 354.1 ± 28.2 | 385.0 ± 53.6 | 235.3 ± 25.3 | 0.001 | |
| PCae_C36:3 | 96.2 ± 15.0 | 108.6 ± 10.5 | 120.5 ± 17.9 | 65.2 ± 8.9 | 0.001 | |
| PCae_C38:1 | 23.7 ± 4.2 | 29.7 ± 2.2 | 29.9 ± 5.0 | 18.7 ± 1.2 | 0.002 | |
| PCae_C38:2 | 62.5 ± 9.7 | 74.9 ± 6.3 | 78.0 ± 13.3 | 50.7 ± 5.2 | 0.004 | |
| PCae_C38:3 | 27.3 ± 3.6 | 33.8 ± 3.9 | 34.7 ± 5.8 | 18.5 ± 1.8 | 0.000 | |
| PCae_C38:4 | 58.2 ± 7.5 | 69.0 ± 7.3 | 70.5 ± 9.1 | 38.1 ± 4.0 | 0.000 | |
| PCae_C40:4 | 10.1 ± 1.5 | 11.7 ± 1.5 | 12.8 ± 2.0 | 7.6 ± 0.5 | 0.002 | |
| PCae_C40:5 | 26.3 ± 4.4 | 30.0 ± 2.5 | 33.3 ± 4.2 | 16.5 ± 1.5 | 0.000 | |
| SM_C16:1 | SMs | 29.5 ± 2.8 | 35.3 ± 3.4 | 35.0 ± 3.8 | 25.5 ± 2.1 | 0.002 |