Fig. 1.

Expression of Rar, Rxr, and Raldh isoforms in rat GCs. GCs from DES-primed immature rats were cultured for 24 h. Levels of Rarα, Rarβ, Rarγ, Rxrα, Rxrβ, Rxrγ, Raldh1, Raldh2, and Raldh3 mRNAs (in triplicate) were determined by RT-PCR analysis. (A) Representative autoradiograms illustrating the expression of Rar, Rxr, and Raldh mRNAs using 2 μg of total RNA. (B) Results show the corresponding intensities of Rar, Rxr, and Raldh bands normalized with the corresponding Rpl19 bands. Data in the bar graphs represent the means±SEMs from three independent experiments.