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Norcantharidin (NCTD) induced cell cycle arrest of SK-N-SH cells. A, SK-N-SH cells were exposed to various concentrations of NCTD (0-40 μmol/L) for 24 hours followed by analysis of cell cycle by flow cytometry. B, Western blot analysis of cell cycle-associated protein extracts obtained from SK-N-SH cells treated with different concentrations of NCTD. C, Cyclin family members were tested after NCTD treatment as described.
