Figure 3.

Induction of apoptosis and reduction in mitochondrial membrane potential in SK-N-SH cells by norcantharidin (NCTD). A, Norcantharidin-induced apoptosis determined by annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining. B, The change in mitochondrial membrane potential after 0, 5, 10, 20, or 40 μmol/L of NCTD explosion was evaluated by flow cytometry. C, Fluorescence microscopy was used to determine the mitochondrial membrane potential after different concentration (0, 20, and 40 μmol/L) of NCTD treatment. Data shown are representative of 3 different experiments.