Norcantharidin (NCTD) changed expression levels of Bcl-2 family proteins and activated caspase-3/caspase-9/PARP in SK-N-SH cells. A, Norcantharidin inhibited B-cell lymphoma 2 (Bcl-2) family members. SK-N-SH cells were treated as described previously, and the effect of NCTD on Bcl-2 family members’ expression level was determined by Western blot. B, Norcantharidin activated caspase-3 and caspase-9. SK-N-SH cells were treated with 0, 5, 10, 20, and 40 μmol/L NCTD for 24 hours. The effects of NCTD on procaspase-3, procaspase-8, procaspase-9, and PARP were evaluated by Western blot. Data represent one of 3 experiments yielding similar results.