Figure 6.

Effects of norcantharidin (NCTD) on intracellular signaling protein expression levels in SK-N-SH cells. A, Norcantharidin activates the AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway in SKNCH cell. SK-N-SH cells were treated with 0, 5, 10, 20, and 40 μmol/L NCTD for 24 hours. The effects of NCTD on AMPK, phosphor–AMP-activated protein (p-AMPK), AKT, phosphor-protein kinase B (p-AKT), and mTOR expression levels were evaluated by Western blotting. B, Norcantharidin activates the c-Jun NH2-terminal kinases (JNK)/c-Jun pathway in SK-N-SH cells. After treatment, SK-N-SH cells were subjected to Western blotting for JNK, phosphor-c-Jun NH2-terminal kinases (p-JNK), c-Jun, and phosphor-c-Jun (p-c-Jun). Both experiments were individually performed 3 times and present representative data.