Figure 3.

MicroRNA (miRNA)-96 decreases the expression of PDCD4. A, The sequences in the 3′UTR of PDCD were predicted to potentially bind to miRNA-96. The red nucleotides were mutated to their complementary nucleotides. B, Western blot analysis shows that overexpression of microRNA-96 inhibits the protein level of PDCD in U87 and U251 cells. C, Real-time polymerase chain reaction (PCR) analysis shows that overexpression of microRNA-96 inhibits the mRNA level of PDCD in U87 and U251 cells. D, The luciferase reporter activity assay shows that microRNA-96 suppresses luciferase activity in the wild-type UTR but does not suppress activity in the mutant UTR.