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. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Cell Rep. 2017 Sep 5;20(10):2396–2407. doi: 10.1016/j.celrep.2017.08.040

Figure 2. OTU activity in transfected cells.

Figure 2

To assess the DUB and deISGylase activity of the OTU mutants, Huh7 cells were co-transfected with plasmids expressing HA-Ub or V5-ISG15, Ube1L, UbcH8 and HERC5. Cell lysates were harvested 2 days post transfection, and proteins analyzed for Ub- or ISG15-conjugates. The OTU activities of both the single OTU domain (A) and the OTU in the context of the complete L protein were assessed by western blot (B). (C) The cellular levels of CCHFV L protein, co-expressed with HA-Ub or ISG15, were analyzed in Huh7 cells by western blot.