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. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Cell Rep. 2017 Sep 5;20(10):2396–2407. doi: 10.1016/j.celrep.2017.08.040

Figure 3. Effects of OTU mutations on immune suppression.

Figure 3

To assess the effect of OTU on the RIG-I-mediated IFN response, HEK293T cells were transfected with plasmids expressing the CCHFV OTU domain, the constitutively active RIG-I CARDs to induce IFN-β expression and a firefly luciferase reporter gene under control of the promoter of IFN-β (A), or containing binding sites for IRF3 (B) or NF-κB (C). Data are presented as mean ± SD of 3 biological replicates and * indicate statistical significance of p<0.05 between the wild-type OTU -and the individual mutants. OTU expression and activation of the RIG-I mediated immune response was confirmed by western blot analysis (D).