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. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Cell Rep. 2017 Sep 5;20(10):2396–2407. doi: 10.1016/j.celrep.2017.08.040

Figure 5. General levels of ubiquitinated/ISGylated proteins in CCHFV-infected cells.

Figure 5

(A) Western blot of ubiquitinated and ISGylated proteins in CCHFV-infected cells. Huh7 cells were infected with CCHFV at an MOI of 5. Cell lysates were harvested 24h post infection, separated by SDS-PAGE and probed for total Ub or K48- and K63-linked poly-Ub chains. (B) To assess protein ISGylation in infected cells, IFN β (1000 IU/ml) was added to the cell culture media 4 h post infection. Conjugation of ISG15 was visualized using western blotting.