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. 2017 Jul 31;12(8):e1356532. doi: 10.1080/15592324.2017.1356532

Figure 1.

Figure 1.

Subcellular localization of OsERS1 and OsETR2. (a) Schematic representation of the gene constructs for transient expression in onion cells. Expression is driven by the CaMV 35S promoter in the GFP control, at the 5′-end of OsERS1 cDNA (360 bp from start codon) and OsETR2 cDNA (480 bp from start codon) to fuse with GFP cDNA at the 3′ end, including the stop codon. (b) Expression of pGFP (control) in onion cells. (c) Expression of pERS1(N-120)–GFP in onion cells. (d) Expression of pERS1(N-120)–GFP in onion cells in hypertonic solution (0.5 M CaCl2). (e) Expression of pETR2(N-160)–GFP in onion cells. (f) Expression of pETR2(N-160)–GFP and pERS1(N-120)–GFP in onion cells with rhodamine B hexylester staining. (R) Filtered image specific for rhodamine B hexylester observation, (G) filtered image for GFP observation and (R+G) image with 2 filtered outputs, (R) and (G). White bars at the lower-right hand corners of each image represent the actual length as indicated.