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. Author manuscript; available in PMC: 2018 Aug 2.
Published in final edited form as: Neuron. 2017 Jul 20;95(3):608–622.e5. doi: 10.1016/j.neuron.2017.06.048

Figure 1. Mena interacts with RNA binding proteins and cytosolic mRNAs.

Figure 1

A. Panels show western blots, probed with antibodies to the indicated proteins, of Mena and IgG2a isotype control IPs and of 5% input lysate. B. Proteins enriched in Oligo(dT) pulldowns, analyzed by western blot probed with antibodies to Mena and to positive control RBPs, FMR1 and MBNL1, as indicated (Input is 5% of total lysate). C. Schematic representation of the modified HITS-CLIP protocol. E15.5 mouse brain tissues were triturated and UV-crosslinked to preserve RNP-complexes, and then homogenized in mild lysis buffer to generate lysates for Mena-IP. Co-IPed RNA was subsequently isolated and sequenced. D. Gene-Set-Enrichment-Analysis (REACTOME) of the mRNAs identified through Mena HITS-CLIP. E. qPCR validation of several mRNAs that specifically associated with Mena. Mena null brains were used as experimental controls. The graph represents relative mRNA enrichment of Mena-Associated mRNAs between the wt and mve samples ± StDEV (Student’s T test p*<0.05). F. Peaks in the 3’UTRs of mena and dyrk1a.