Figure 3. Procedures to load animals to the chambers.

A. Cleaned chip ready for the assay. B. Distribution of food droplets into each chamber. C. Early- to mid-L4 animals loaded into each droplet, leaving two as blank controls. D. Spreading of movie food across each chamber surface aid before adding the coverslip will decrease the odds of having two animals trapped in the same chamber. E. Coverslip is now in place, which traps each animal into a single chamber. Scale bars = 1 mm. F. The cover glass-chip-slide sandwich assembled for imaging. Scale bar = 5 mm.