(A) A false-color transmission electron micrograph and deep-etched
freeze-fractured image of the pyrenoid highlight the pyrenoid tubules, starch
sheath and pyrenoid matrix where the principal carbon fixing enzyme, Rubisco, is
located. Images courtesy of Moritz Meyer, Ursula Goodenough and Robyn Roth.
(B) Proteins showing various localization patterns within the pyrenoid are
illustrated. Scale bar: 5 μm.
(C) Confocal sections distinguish different localization patterns within the
pyrenoid. Each end panel is a space-filling reconstruction. Scale bars: 2
μm.
(D) Dual tagging refined the spatial distribution of proteins in the pyrenoid.
Scale bar: 5 μm.
(E) A proposed pyrenoid model highlighting the distinct spatial
protein-containing regions.