Figure 4.

Phenotypic Differences between Wild-Type, prM_100,101, and E₃₁₅ Viruses

(A) C6/36 cells were infected with a mixture containing WT virus and the prM_100,101 (yellow) or E₃₁₅ (red) mutant viruses. The replication index was measured as the log₂ value of relative levels of mutant and WT genomes in the supernatant at 3–5 d.p.i., compared with the corresponding ratios in the input. A negative index indicates that the replicative ability of mutant virus is lower than WT virus.

(B) U937-DC-SIGN cells were mock-infected (mock, blue) or infected with WT (aqua), prM_100,101 (yellow), and E₃₁₅ (red) viruses, fixed, and stained with anti-E (left panel) and anti-NS3 (right panel) antibodies at various time points post infection. All data points for (A and B) represent the average of two independent experiments, mean ± SD.