KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-GFP | Roche | Cat# 11814460001; RRID: AB_390913 |
| Mouse anti-CLTC | Santa Cruz | Cat# sc-12734; RRID: AB_627263 |
| Goat anti-VPS35 | Novus Biologicals | Cat# NB100-1397; RRID: AB_2257186 |
| Mouse anti-FLAG (M1) | Sigma | Cat# F-3040; RRID: AB_439712 |
| Rabbit anti-TOM1 | Abcam | Cat# Ab170928 |
| Rabbit anti-WWP2 | Abcam | Cat# AB103527; RRID: AB_10710285 |
| Mouse anti-GAPDH | EMD Millipore | Cat# MAB374; RRID: AB_2107445 |
| Donkey anti-goat (680 conjugate) | LI-COR | Cat# 926-68074; RRID: AB_10956736 |
| Donkey anti-rabbit (680 conjugate) | LI-COR | Cat# 926-68073; RRID: AB_10954442 |
| Donkey anti-mouse (800 conjugate) | LI-COR | Cat# 926-32212; RRID: AB_621847 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Streptavidin Agarose Resin | Thermo Scientific/Pierce | Cat# PI-20353 |
| Biotin Phenol (Biotin Tyramide) | Berry & Associates | Cat# BT 1015 |
| Biotin Phenol (Biotin Tyramide) | Adipogen | Cat# CDX-B0270 |
| Deposited Data | ||
| Shotgun proteomics RAW and analyzed data | This study | http://www.ebi.ac.uk/pride/archive/projects/PXD005758 |
| Targeted proteomics RAW and analyzed data | This study | https://panoramaweb.org/labkey/GPCR-APEX.url |
| Experimental Models: Cell Lines | ||
| Human HEK293 Cells | ATCC | CRL-1573 |
| Recombinant DNA | ||
| pcDNA3.1: FLAG-B2AR-382-APEX2 | This study | N/A |
| pcDNA3.1: FLAG-DOR-APEX2 | This study | N/A |
| pcDNA3: CYTO-APEX2 | This study | N/A |
| pcDNA3: ENDO-APEX2 | This study | N/A |
| pcDNA3: PM-APEX2 | This study | N/A |
| pGLO-20F | Promega | Cat# E1171 |
| Sequence-Based Reagents | ||
| siRNA hWWP2 | QIAGEN | Cat# SI05083547 |
| siRNA hTOM1 | QIAGEN | Cat# SI03095344 |
| Primers listed in Table S6 | IDT | N/A |
| Software and Algorithms | ||
| Java TreeView 1.1.6r4 | Saldanha, 2004 | http://jtreeview.sourceforge.net |
| Gene Cluster 3.0 | de Hoon et al., 2004 | http://bonsai.hgc.jp/~mdehoon/software/cluster/ |
| PANTHER Overrepresentation Test of Cellular Components (release 20160715) | Ashburner et al., 2000; Gene Ontology Consortium, 2015 | http://geneontology.org/page/go-enrichment-analysis |
| MaxQuant | Cox and Mann, 2008 | http://www.coxdocs.org/doku.php?id=:maxquant:start |
| MSstats | Choi et al., 2014 | http://msstats.org |
| SAINTexpress | Choi et al., 2011 | http://saintapms.sourceforge.net/ |
| Skyline | MacLean et al., 2010 | https://skyline.ms/project/home/software/Skyline/begin.view |
| Prism 6.0h | GraphPad | https://www.graphpad.com/scientific-software/prism/ |