Abstract
Methamphetamine (MA) is strong central nervous system stimulant knowns, both in humans and in animals. However, there have been no previous studies which investigate brain alterations by period after injection in acute model. So this study aimed to estimate the alteration of the resting-state functional connectivity (rsFC) of the caudate nucleus(CN), anterior cingulate cortex (ACC), and superior frontal gyrus (SFG) as regions of interest(ROIs) after MA administration in none human primate.
16 macaca fascicularis (6months-11years) were evaluated once (baseline) before MA injection and 7 times (12hours-4weeks) after MA injection (2mg/kg, intra-muscle). The MRI data were acquired using a 3T MRI system (Philips Achiva), and the resting-state images were preprocessed and analyzed with the statistical parametric mapping toolbox (SPM8). To examine the changed rsFC of seeds, we used a seed-based correlation approach. Results were considered statistically significant if they exceeded the uncorrected p < .005 with an extent threshold of 10 voxels.
The rsFC from the left CN to the middle temporal gyrus was decreased steadily for period of 4 weeks after MA injection. In addition, from the left SFG to the ACC showed decreased rsFC in the same period. The observations of dysfunction of temporo-striatal and fronto-cingulate circuit in acute MA nonhuman primate model suggest that acute effect of MA induce functional abnormality in the brain.
Acknowledgements
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2014M3A9B6070246).