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. 2017 Sep 27;12(9):e0185441. doi: 10.1371/journal.pone.0185441

Fig 1. Class II and IV Hdac expression during osteoclast differentiation.

Fig 1

BMM cells from C57BL/6 mice were cultured in M-CSF only (day zero) or in M-CSF plus RANKL (day two and day four) to stimulate osteoclast differentiation. Class II and IV Hdac RNA expression (A) on day zero, day two and day four of osteoclast differentiation. qRT-PCR data shown are the mean of three independent experiments. * p <0.05 comparing vs. day zero, ns = not significant. Representative protein expression (B) of class II and IV HDACs on each day of osteoclast differentiation. Displayed relative expression values are HDAC expression relative to the normalized expression on day zero as calculated by Image Lab Software (BioRad). In the case of HDAC9 no band was identified for day zero so data is expressed relative to day one. The same lysates were analyzed for HDAC9 and MITR expression and therefore, have the same actin loading control.