Table 1. Precision analysis of the lipL32 real-time PCR assay.

Nominal Conc (GEq/rxn)	Repeatabilitya				Reproducibilityb
	Mean Conc. (GEq/rxn)	Mean Ctc	SD (Log10)	CV (%)	Mean Conc. (GEq/rxn)	Mean Ct	SD (Log10)	CV (%)
1	2	38.44	0.17	68.44	2	38.64	0.16	83.80
10	12	36.62	0.11	10.61	12	36.61	0.13	12.51
100	98	33.06	0.07	3.51	111	32.86	0.09	4.28
1,000	935	29.48	0.07	2.20	1,014	29.38	0.09	3.12
10,000	10,191	25.91	0.06	1.53	10,261	25.89	0.05	1.28
100,000	103,669	22.50	0.07	1.34	96,961	22.57	0.08	1.53
1,000,000	1,134,389	19.00	0.07	1.10	1,095,684	19.03	0.05	0.90
10,000,000	11,187,790	15.65	0.06	0.86	10,497,559	15.71	0.06	0.88

^aAssessed with 11 replicates tested on the same day.

^bAssessed with 2 replicates tested on 32 different days (n = 64).

^cCycle Threshold.