Fig. S5.

CK1α depletion in human primary keratinocytes induces p21 and KITL up-regulation. Human primary keratinocytes were transfected with CK1α siRNA. (Left) Two days after transfection, cell lysates were analyzed using Western blotting with antibodies as indicated. Total RNAs were isolated and reverse transcribed, and mRNA expression levels were analyzed by qPCR with primer pairs as indicated. (Right) mRNA levels were calculated relative to the scrambled control (Con.) with the housekeeping gene GAPDH as a reference. Data are shown as means ± SD; *P < 0.05.