Fig. 1.

Treg-specific Il27ra^−/− mice. (A) Generation of neo-free Il27ra-floxed mice by the two-loxP and two-frt strategy. (B) IL-27Rα expression of naive (T_N), memory phenotype (T_M), and Foxp3⁺ Tregs (T_reg) in Treg-specific Il27ra^−/− (Treg^ΔIl27ra) and littermate control (Treg^WT) mice. (C) Foxp3⁺ and conventional CD4 T cells were sorted from Treg^ΔIl27ra mice and in vitro-stimulated with plate-coated anti-CD3/CD28 Abs in the presence of IL-27. Fold increases of the indicated genes were determined by qPCR analysis. (D and E) Lymph node cells were stained for CD4, CD8, CD44, and CD62L expression. Proportions of naive and effector/memory phenotype CD4 T cells (D) and surface phenotypes of Tregs (E) were examined. All of the experiments shown were repeated three times, and similar results were observed.