Fig. 4.
IL-27 signaling in Tregs controls Treg stability. (A) Groups of Rosa26CAG-tdTomato TregΔIl27ra and Rosa26CAG-tdTomato TregWT mice were induced for EAE. Clinical score was daily monitored. (B) Mice were killed 21 d post induction. YFP and tdTomato reporter expression of CD4 gated CNS cells is shown. (C) Absolute numbers of Tregs and exTregs in the CNS were calculated. (D) CNS cells were ex vivo-stimulated and intracellular IFNγ and IL-17 expression of Tconv, exTreg, and Tregs was determined by FACS analysis. (E) Absolute numbers of IFNγ- and IL-17–producing exTregs were calculated. The data shown are representative of two independent experiments (n = 6 ∼ 7). *P < 0.05; **P < 0.01.