Fig. 7.

One methylated EcoRI site is sufficient for transformation. (A) Diagram of the plasmids used. EcoRI sites (GAATTC) are in red, and CtsM sites (RAATTY) that are not methylated by M.EcoRI are shown in green. The exact sites of the EcoRI sites are indicated for pJMB204. The plasmids are identical except for the location and number of EcoRI sites. The plasmid backbone is indicated in black, and regions homologous to C. jejuni are indicated in blue. The antibiotic cassette is in yellow. (B) WT cells (blue) or ctsM::kan cells (yellow) were transformed with different plasmids as indicated, and transformation efficiency was measured as before. n.d. indicates that colonies were not detected. Error bars indicate SD. *P < 0.01; **P < 0.01, Kruskal–Wallis test with Dunn’s multiple-comparison posttest.