Fig. 8.

Methylated RAATTY promotes DNA uptake. DRH212 cells were incubated with radiolabled pJMB204 that had been methylated with M.EcoRI (+MT) or mock-methylated (−MT) for 1 h. The methyltransferase experiments were initiated with 5 ng/µL of DNA. Cells were centrifuged, washed, split, and either mock-treated or treated with DNase. Mock-treated cells contain the bound DNA (blue), and DNase-treated cells contain DNA that has been transported into the cells (yellow); 100 ng of DNA was used in each experiment. After treatment, cells were washed and resuspended in scintillation fluid. Total cpm was measured using a scintillation counter. Data are representative of the experiment repeated three times. Error bars indicate SD of three technical replicates. *P < 0.05, two-way ANOVA and Tukey’s posttest.