Table S2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source |
| Bacteria | ||
| E. coli | ||
| DH5α | supE44 ΔlacU169 (Ф80lacZΔM15) hsDr17 recA1 endA1gyrA96 thi-1 relA1 | Lab strain |
| GM2163 | dam-, dcm-, camR | New England BioLabs |
| DH5α pRK212.1 | Contains conjugative plasmid for transferring DNA into Campylobacter | (46) |
| C. jejuni | ||
| 81–176 | Clinical isolate | (47) |
| 11168 | Clinical isolate | |
| DRH153 | 81–176 astA::aphA3; Kanr | (48) |
| DRH154 | 81–176 astA::cat, CmR | (48) |
| DRH212 | 81–176 rpsLSm (this strain is referred to as “wild type” throughout) | (48) |
| JMB200 | DRH212 ctsM::kan, KanR | This study |
| JMB202 | DRH212 NAR | This study |
| JMB204 | DRH212 ctsM::cm, CmR | This study |
| Plasmids | ||
| pUC19 | AmpR | New England BioLabs |
| pEC0102 | pRY112 derivative with cat promoter | (29) |
| pJMB200 | pUC19 ctsM::kan, KanR | This study |
| pJMB201 | pUC19 ctsM::cm, CmR | This study |
| pJMB202 | pUC19 astAkan, KanR | This study |
| pJMB203 | pUC19 astAcm, CmR | This study |
| pJMB204 | pJMB203 + new EcoRI site at 4053, CmR | This study |
| pJMB205 | pJMB203 only 1 EcoRI site in MCS, CmR | This study |
| pJMB206 | pJMB203 only 1 EcoRI, in astA, CmR | This study |
| pJMB207 | pJMB203 only 1 EcoRI site at 4053 (away from insert), CmR | This study |
| pJMB208 | pJMB203 no EcoRI sites, CmR | This study |