Table S3.

Primers used in this study

Primer no.	Primer name	Sequence (5′-3′)	Description
145	0240 kan/CM GA 1	ATT CGA CGT CGG TAC CCG GGT GTG TTG GCG ATG ATG GC	Used to make 0240::kan and 0240::cm insertional mutants
146	0240 Kan GA 2	TCA TTT TAG CCA TAA TTT AAA ACC TTT AAA AGC CTT ATC CAC	Used to make 0240::kan insertional mutant
147	0240 Kan GA 3	GGT TTT AAA TTA TGG CTA AAA TCA CAA TAT CAC	Used to make 0240::kan insertional mutant
148	0240 Kan GA 4	AGC ACA AAA TCT AAA ACA ATT CAT CCA GTA AAA TAT AAT ATT TTA TTT TTC	Used to make 0240::kan insertional mutant
149	0240 Kan GA 5	ATT GTT TTA GAT TTT GTG CTA AAA TTA TAT TTA TAA AAA GG	Used to make 0240::kan insertional mutant
150	0240 Kan/CM GA 6	CAA GCT TGC ATG CCT GCA GGC TTT TAT TGA GAC TAA TCT TAC TCA TTT TTA AAA C	Used to make 0240::kan and 0240::cm insertional mutants
151	0240 CM GA 2	TTA ACT TGG AAA TTT AAA ACC TTT AAA AGC CTT ATC CAC	Used to make 0240::cm insertional mutant
152	0240 CM GA 3	GGT TTT AAA TTT CCA AGT TAA TTG CGT GAT ATA G	Used to make 0240::cm insertional mutant
153	0240 CM GA 4	AGC ACA AAA TTG CGC CCT TTA CTT CCT AAA G	Used to make 0240::cm insertional mutant
154	0240 CM GA 5	AAA GGG CGC AAT TTT GTG CTA AAA TTA TAT TTA TAA AAA GG	Used to make 0240::cm insertional mutant
183	pUC19astA F	GGC ACT TTT GGC GGG TTC TAC C	Used to create astAkan PCR fragment
184	pUC19astA R	GGA TCA TAA TCA ACG CTA TTT ACA TGA GCC C	Used to create astAkan PCR fragment
185	M13 F	TGT AAA ACG ACG GCC AGT	Used to create M13 PCR product
186	M13 R	CAG GAA ACA GCT ATG ACC	Used to create M13 PCR product
187	astA1 F GA	AGT GAA TTC GAG CTC GGT ACC CGG GAT GAG ACT TAG CAA AAC TCT TTG	Used to make pUC19astAkan and pUC19astAcm
188	astA1 cm R GA	CAA TTA ACT TGG AAA CAA CAT TAC CAT TCT CAT C	Used to make pUC19astAcm
189	CM F GA	TGG TAA TGT TTC CAA GTT AAT TGC GTG ATA TAG	Used to make pUC19astAcm
190	CM R GA	ATC TCC AAT CAT CAA TGC GCC CTT TAG TTC C	Used to make pUC19astAcm
191	astA 2 CM F GA	TAA AGG GCG CAT TGA TGA TTG GAG ATT GTA TGA G	Used to make pUC19astAcm
192	astA 2 R GA	TAC GCC AAG CTT GCA TGC CTG CAG GTT ATT TTT TAG GAT TGA ATG CTT GAT C	Used to make pUC19astAkan and pUC19astAcm
193	astA 1 Kan R GA	TCA TTT TAG CCA TTC ATT TTA GCC ATA ACA ACA TTA C	Used to make pUC19astAkan
194	Kan F GA	ATG GCT AAA ATG AAT GGC TAA AAT GAG AAT ATC AC	Used to make pUC19astAkan
195	Kan R GA	ATC TCC AAT CAT CCT AAA ACA ATT CAT CCA GTA AAA TAT AAT ATT TTA TTT TC	Used to make pUC19astAkan
196	astA2 Kan F GA	TGA ATT GTT TTA GGA TGA TTG GAG ATT GTA TGA G	Used to make pUC19astAkan
197			Used for binding and uptake assay to amplify off kan fragment
198			Used for binding and uptake assay to amplify off kan fragment