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. 2017 Sep 5;114(38):10268–10273. doi: 10.1073/pnas.1704143114

Fig. 4.

Fig. 4.

ALGERNON promotes neurogenesis in the dentate gyrus of the adult mouse hippocampus (A–C) and rescues impaired neurogenesis in Ts1Cje mice (D–F). (A, Upper) Experimental timeline for assessing NSC proliferation after EdU and BrdU injections following ALGERNON treatment via drinking water. (A, Lower) Representative image of proliferating cells at day 4, the survival of these cells 1 wk later (EdU, red), and proliferating cells at day 10 (BrdU, green). (Scale bar, 100 μm.) (B and C) Quantified BrdU-positive cells and EdU-positive cells in the dentate gyrus of the hippocampus in each group, respectively (n = 3). (D, Upper) Experimental timeline for assessing NSC proliferation and differentiation after EdU and BrdU injections following s.c. ALGERNON administration. (D, Lower) Representative image of newly generated immature neurons positive for DCX (red) and a cell proliferation marker (EdU, green) in the dentate gyrus of the hippocampus. (Scale bar, 100 μm.) (E–G) Quantification of BrdU-positive cells (E), EdU-positive cells (F), and DCX-positive cells (G). n = 10 per group. (H, Upper) Experimental timeline for assessing NSC proliferation and differentiation after BrdU injections following ALGERNON treatment. (H, Lower) Representative images of newly generated immature neurons positive for DCX (green) and a proliferation marker (BrdU, red) in the dentate gyrus of the hippocampus from WT and trisomy mice. (Scale bar, 100 μm.) (I and J) Quantified BrdU-positive cells and BrdU/DCX double-positive cells in each group, respectively (n = 8). F(3,28) = 6.99, P = 0.0015 (I); F(3,28) = 6.88, P = 0.0014 (J). *P < 0.05.