Fig. 2.

The gonadotrope precursor TET1 isoform is N-terminally truncated, found in other differentiated tissues, and uses an alternate promoter that binds liganded ESR1 and AR. (A) RT-PCR analyses for the Tet1 canonical exon 1, the alternative exon “1.5,” and the canonical exon 3 in mouse ESCs, αT3-1 and LβT2 cell lines, and gDNA, with Gapdh control. (B) qPCR of αT3-1 cells and primary gonadotropes from immature mice using the same primer sets and quantified by using a standard curve of gDNA, shown relative to levels of exon 3 (n = 5). (C–G) ChIP analysis for (C) H3K4me3 and (D) H3K27ac in αT3-1(Left) and LβT2 (Right) cells for the region upstream of the canonical exon 1 (−14,665 to −14,572 bp) and exon 1.5 (−53 to +68) or in (E) RNAPII S5p, (F) ESR1, and (G) AR in αT3-1 cells (exposed for 2 h to E₂ or DHT as noted). IP levels are relative to input (n = 3; Figs. S2 and S3).