Fig. S2.

The gonadotrope Tet1 isoform lacks exon 1, which encodes the CXXC domain (Fig. 2). (A) RT-PCR of the mouse brain (C, cerebellum; CC, cerebral cortex; OB, olfactory bulb) or placenta (marked as “P”) or (B) other mouse tissues using the same primer sets. G, GAPDH; NTC, no template control. (C) RT-PCR was carried out on αT3-1 cells using sets of primers targeting the first three possible exons of Tet1 as shown. (D) 5′RACE was carried out on αT3-1 cell cDNA, with nested primers targeting exon 3 of Tet1. The amplification product of the second round of PCR was resolved by gel electrophoresis, and the ∼500-bp product was identified by sequencing. The actual sequence is shown, with the start of the published sequence underlined.